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一、大鼠PPARd ELISA 試劑盒的產(chǎn)品簡述：
ELISA 品 牌：USCN
ELISA 貨 號：072041r
ELISA英文名：Canine TNFa ELISA Kit
ELISA中文名：大鼠過氧化物酶體增殖物激活受體δ，PPARd ELISAkit
1. 檢測范圍：
2. 靈 敏 度：
3. 適用標(biāo)本：
4. 保存條件：2-8℃
5. 有 效 期：6個月
三、rat PPARd ELISA 試劑盒的產(chǎn)品用途：
產(chǎn)品用途：酶聯(lián)免疫實驗(ELISA實驗)
備 注：僅供參考，請以隨貨紙質(zhì)說明書為準(zhǔn)，更多產(chǎn)品如抗體(antibody)；蛋白(protein)；鱟試劑；內(nèi)毒素工作標(biāo)準(zhǔn)品；內(nèi)毒素指示劑；無熱原檢查用水；無熱原吸咀；紫外燈；色譜儀；色譜柱等更詳細信息請登錄上海武昊公司網(wǎng)站禁發(fā)網(wǎng)址wuhaosh禁發(fā)網(wǎng)址 或致電咨詢。
ELISA實驗前，血清、血漿、組織、細胞培養(yǎng)上清等樣品的采集及處理
Serum- Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximately 1000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS(0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice(Micro Tissue Grinders woks, too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediately or aliquot and store at ≤-20oC.
Cell culture supernates - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
